Recombinant Mouse Ighg1 protein

• Cat.No.: Ighg1-5543M
• Product Overview: Recombinant Mouse Ighg1 protein(P01868)(Val98-Lys324) was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Non
• Protein Length: Val98-Lys324
• Tag: Non
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Mouse FcRn, His Tag captured on CM5 Chip via anti-his antibody can bind Mouse IgG1 Fc, No Tag with an affinity constant of 15.09 nM as determined in SPR assay (Biacore T200).
• Molecular Mass: The protein has a predicted MW of 25.7 kDa. Due to glycosylation, the protein migrates to 34-36 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.