Recombinant Mouse Ighg3 protein

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Recombinant Mouse Ighg3 protein

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Cat.No. : Ighg3-7302M
Product Overview : Recombinant Mouse Ighg3 (Glu97-Lys329) protein carried no tag and was expressed in human 293 cells (HEK293).
Description : Immunoglobulin G3 (IgG3) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. Ig gamma-3 chain C region (IgG3-Fc/IGHG3) contains two constant regions of IgG3 H chain (CH2, CH3).
Source : HEK293
Species : Mouse
Predicted N Terminal : Glu97
Form : Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 26.2 kDa. The protein migrates as 33-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : Glu97-Lys329
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of Ighg3-7302M :

Mouse IgG3 Fc, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Gene Name : Ighg3
Official Symbol : Ighg3
Synonyms : IgG3
Gene ID : 380795
UniProt ID : Q6KAM2

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