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Recombinant Mouse Itk Protein, GST & His-tagged

Cat.No.: Itk-861M
Product Overview: Recombinant mouse ITK / EMT (Q03526) protein kinase domain (Arg 351-Leu 619) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Source: Baculovirus-Insect Cells
Species: Mouse
Tag: GST & His
Predicted N Terminal: Met
Form: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 7.5, 10% gly, 0.5mM GSH1, 5%~8% trehalose and mannitol.
Bio-activity: No Kinase Activity
Molecular Mass: The secreted recombinant mouse ITK /GST chimera consists of 506 amino acids and has a calculated molecular mass of 58.4 kDa. The recombinant protein migrates as an approximately 58 kDa band in SDS-PAGE under reducing conditions.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >95 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: Itk IL2 inducible T cell kinase [ Mus musculus ]
Official Symbol: Itk
Synonyms: ITK; IL2 inducible T cell kinase; tyrosine-protein kinase ITK/TSK; kinase EMT; kinase TLK; T-cell-specific kinase; IL2-inducible T-cell kinase; IL-2-inducible T-cell kinase; interleukin-2-inducible T cell kinase; Emt; Tsk; Tcsk;
Gene ID: 16428
mRNA Refseq: NM_010583
Protein Refseq: NP_034713
MIM:
UniProt ID:
Pathway: Adaptive Immune System, organism-specific biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; Chemokine signaling pathway, organism-specific biosystem; Chemokine signaling pathway, conserved biosystem; Generation of second messenger
Function: ATP binding; kinase activity; metal ion binding; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; phospholipid binding; protein binding; protein kinase activity; protein tyrosine kinase activity; transferase activity; transferas

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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