| Source : |
HEK293 |
| Species : |
Mouse |
| Tag : |
mFc |
| Form : |
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5 with trehalose as protectant. |
| Bio-activity : |
Immobilized Human VEGF165, premium grade at 2 μg/mL (100 μL/well) can bind Mouse VEGF R2 Protein, Mouse IgG2a Fc Tag with a linear range of 1-31 ng/mL. Immobilized ActiveMax Mouse VEGF120, Tag Free at 2 μg/mL (100 μL/well) can bind Mouse VEGF R2 Protein, Mouse IgG2a Fc Tag with a linear range of 1-31 ng/mL. Immobilized ActiveMax Mouse VEGF164, Tag Free at 2 μg/mL (100 μL/well) can bind Mouse VEGF R2 Protein, Mouse IgG2a Fc Tag with a linear range of 2-31 ng/mL. |
| Molecular Mass : |
This protein carries a mouse IgG2a Fc tag at the C-terminusThe protein has a calculated MW of 110.1 kDa. The protein migrates as 120-135 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Protein Length : |
Ala 20 - Glu 762 |
| Endotoxin : |
Less than 0.1 EU per μg by the LAL method. |
| Purity : |
>95% as determined by SDS-PAGE. |
| Storage : |
For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
