Active Recombinant Mouse Lag3 protein, His-tagged, Low Endotoxin

• Cat.No.: Lag3-1083M
• Product Overview: Recombinant Mouse Lag3 protein(Q61790)(23-442 aa), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 23-442 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Mouse LAG3, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Mouse FGL1, hFc Tag with the EC50 of 0.11μg/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 46.2 kDa. Due to glycosylation, the protein migrates to 65-70 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE
• Storage: Store at -20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: Lag3 lymphocyte-activation gene 3 [ Mus musculus ]
• Official Symbol: Lag3
• Synonyms: LAG3; lymphocyte-activation gene 3; lymphocyte activation gene 3 protein; Ly66; CD223; LAG-3
• Gene ID: 16768
• mRNA Refseq: NM_008479
• Protein Refseq: NP_032505