Recombinant Mouse Lox protein, His & GST-tagged

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Recombinant Mouse Lox protein, His & GST-tagged

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Cat.No. : Lox-7912M
Product Overview : Recombinant Mouse Lox aa. (Pro207~Tyr411) fused with N-terminal His & GST tag was produced in E. coli cells.
Description : This gene encodes a precursor protein that belongs to the lysyl oxidase family of proteins. The secreted proprotein is proteolytically processed to an active mature peptide and a propeptide. This propeptide is thought to function in tumor suppression by inhibiting the Ras signaling pathway. The active enzyme plays a role in cross-linking of collagen and elastin and is essential for development of cardiovascular and respiratory systems, and development of skin and connective tissue. Alternative splicing results in multiple transcript variants.
Source : E. coli
Species : Mouse
Tag : His & GST
Form : Freeze-dried powder
Molecular Mass : 54kDa as determined by SDS-PAGE reducing conditions.
Protein length : Pro207~Tyr411
Endotoxin : <1.0EU per 1ug (determined by the LAL method)
Purity : >92%
Characteristic : The isoelectric point is 6.1.
Applications : SDS-PAGE; WB; ELISA; IP; CoIP; ReporterAssays; Purification; Amine Reactive Labeling.
Stability : The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Concentration : 200μg/mL
Storage Buffer : 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% Trehalose and Proclin300.
Reconstitution : Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.
SDS-PAGE :

Gene Name : Lox lysyl oxidase [ Mus musculus (house mouse) ]
Official Symbol : Lox
Synonyms : Lox; lysyl oxidase; rrg; TSC-160; AI893619; protein-lysine 6-oxidase; ras excision protein; ras recision
Gene ID : 16948
mRNA Refseq : NM_001286181.1
Protein Refseq : NP_001273110.1
UniProt ID : P28301

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