Active Recombinant Mouse Manf protein

• Cat.No.: Manf-187M
• Product Overview: Active Recombinant Mouse Manf protein(Q9CXI5)(22-179) was expressed in E.coli.

Specification

• Species: Mouse
• Source: E.coli
• Tag: Non
• Protein Length: 22-179
• Form: Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
• Bio-activity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 10 μg/ml, corresponding to a specific activity of > 100 IU/mg.
• Molecular Mass: Approximately 18.2 kDa, a single non-glycosylated polypeptide chain containing 158 amino acids.
• AASequence: LRPGDCEVCI SYLGRFYQDL KDRDVTFSPA TIEEELIKFC REARGKENRL CYYIGATDDA ATKIINEVSK PLAHHIPVEK ICEKLKKKDS QICELKYDNQ IDLSTVDLKK LRVKELKKIL DDWGEMCKGC AEKSDYIRKI NELMPKYAPK AASARTDL
• Endotoxin: Less than 0.1 EU/µg of rMuMANF as determined by LAL method.
• Purity: >98% by SDS-PAGE and HPLC analyses.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Gene Information

• Gene Name: Manf mesencephalic astrocyte-derived neurotrophic factor [ Mus musculus ]
• Official Symbol: Manf
• Synonyms: MANF; mesencephalic astrocyte-derived neurotrophic factor; arginine-rich protein; arginine-rich, mutated in early stage tumors; Armet; D17914; AA407711; AA408789; AA673178; D18Mgi17; 3230402M22Rik
• Gene ID: 74840
• mRNA Refseq: NM_029103
• Protein Refseq: NP_083379