Active Recombinant Mouse Manf protein

Species :	Mouse
Source :	E.coli
Tag :	Non
Protein Length :	22-179
Form :	Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
Bio-activity :	Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 10 μg/ml, corresponding to a specific activity of > 100 IU/mg.
Molecular Mass :	Approximately 18.2 kDa, a single non-glycosylated polypeptide chain containing 158 amino acids.
AASequence :	LRPGDCEVCI SYLGRFYQDL KDRDVTFSPA TIEEELIKFC REARGKENRL CYYIGATDDA ATKIINEVSK PLAHHIPVEK ICEKLKKKDS QICELKYDNQ IDLSTVDLKK LRVKELKKIL DDWGEMCKGC AEKSDYIRKI NELMPKYAPK AASARTDL
Endotoxin :	Less than 0.1 EU/µg of rMuMANF as determined by LAL method.
Purity :	>98% by SDS-PAGE and HPLC analyses.
Storage :	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution :	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Gene Name	Manf mesencephalic astrocyte-derived neurotrophic factor [ Mus musculus ]
Official Symbol	Manf
Synonyms	MANF; mesencephalic astrocyte-derived neurotrophic factor; arginine-rich protein; arginine-rich, mutated in early stage tumors; Armet; D17914; AA407711; AA408789; AA673178; D18Mgi17; 3230402M22Rik;
Gene ID	74840
mRNA Refseq	NM_029103
Protein Refseq	NP_083379