Recombinant Mouse Mapkapk2 protein, His & T7-tagged

• Cat.No.: Mapkapk2-6988M
• Product Overview: Recombinant Mouse Mapkapk2 aa. (Arg117~Asp337 (Accession # P49138)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Source: E. coli
• Species: Mouse
• Tag: His&T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 29.1kDa
• Protein length: Arg117~Asp337 (Accession # P49138)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.9.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: Mapkapk2 MAP kinase-activated protein kinase 2 [ Mus musculus (house mouse) ]
• Official Symbol: Mapkapk2
• Synonyms: Mapkapk2; MAP kinase-activated protein kinase 2; MK2; MK-2; Rps6kc1; AA960234; MAPKAP-K2; MAP kinase-activated protein kinase 2; MAPK-activated protein kinase 2; MAPKAP kinase 2
• Gene ID: 17164
• mRNA Refseq: NM_008551.2
• Protein Refseq: NP_032577.1
• UniProt ID: P49138

Reviews

04/15/2022

The lab product sets a new standard for quality. Delivery was speedy, and the customer service team was exceptionally helpful.

11/23/2020

Reliable product, friendly customer service, and efficient delivery.

08/06/2019

Precision in quality, responsive customer service, and fast, secure delivery

Q&As

What structural features of MAPKAPK2 are critical for its kinase activity, and how do these features influence its interactions with substrates and regulatory partners?

MAPKAPK2's kinase activity is influenced by its structural features, including the catalytic domain. These features determine substrate interactions and regulatory partnerships, providing insights into its molecular functions.

What is known about the role of MAPKAPK2 in the immune response, particularly in the regulation of cytokine production and immune cell functions?

MAPKAPK2 plays a role in the immune response by regulating cytokine production and immune cell functions. It influences the activity of transcription factors involved in immune-related gene expression.

How does MAPKAPK2 contribute to cellular motility, and what molecular mechanisms are involved in its regulation of the cytoskeleton and cell migration?

MAPKAPK2 regulates cellular motility by influencing the cytoskeleton and cell migration. It phosphorylates proteins involved in cytoskeletal dynamics, impacting cell motility and invasive behavior.

Can you elaborate on the regulatory mechanisms controlling MAPKAPK2 activation and its substrate specificity?

MAPKAPK2 activation is regulated by p38 MAP kinase through phosphorylation. Its substrate specificity is determined by specific phosphorylation motifs, and its activity is influenced by interacting proteins.

In cancer research, how is MAPKAPK2 implicated in tumor development and progression, and are there potential therapeutic strategies targeting this kinase?

MAPKAPK2 has been associated with tumor development and progression. Understanding its role in cancer biology may lead to the development of targeted therapies, although challenges exist in achieving specificity.