Recombinant Mouse Mcm3 Protein, Myc/DDK-tagged

• Cat.No.: Mcm3-3996M
• Product Overview: Purified recombinant protein of mouse full-length minichromosome maintenance deficient 3 (S, with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 91.5 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Mcm3 minichromosome maintenance complex component 3 [ Mus musculus (house mouse) ]
• Official Symbol: Mcm3
• Synonyms: Mcm3; minichromosome maintenance complex component 3; P; P1; p1.; Mcmd; p1.m; C80350; P1-MCM3; AL033361; DNA replication licensing factor MCM3; DNA polymerase alpha holoenzyme-associated protein P1; mini chromosome maintenance deficient; minichromosome maintenance deficient 3; EC 3.6.4.12
• Gene ID: 17215
• mRNA Refseq: NM_008563
• Protein Refseq: NP_032589
• UniProt ID: P25206