Recombinant Mouse MDH2 Protein, Full Length, N-6×His tagged

• Cat.No.: MDH2-33MFL
• Product Overview: Recombinant mouse Mdh2 protein (Full Length), fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: Full Length
• Description: Enables L-malate dehydrogenase activity. Predicted to be involved in NADH metabolic process; dicarboxylic acid metabolic process; and tricarboxylic acid cycle. Located in membrane. Is expressed in several structures, including alimentary system; nervous system; respiratory system; sensory organ; and urinary system. Human ortholog(s) of this gene implicated in developmental and epileptic encephalopathy 51. Orthologous to human MDH2 (malate dehydrogenase 2).
• Form: Liquid
• Bio-activity: > 800 unit/mg, and is defined as the amount of enzyme that cleaves 1 μmole of oxaloacetate and beta-NADH to L-malate and beta-NAD per minute at pH8.0 at 37 centigrade.
• Molecular Mass: 35.4 kDa (335aa) confirmed by MALDI-TOF
• Purity: > 95% by SDS-PAGE
• Applications: SDS-PAGE, Enzyme Activity
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/mL (determined by absorbance at 280nm)
• Storage Buffer: Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol
• References: 1. Wang L., et al. (2010) Dev Dyn. 239(3):954-64
  2. Chang AK., et al. (2002) J Basic Microbiol. 42(4):238-45.

Gene Information

• Gene Name: Mdh2 malate dehydrogenase 2, NAD (mitochondrial) [ Mus musculus ]
• Official Symbol: MDH2
• Synonyms: MDH2; malate dehydrogenase 2, NAD (mitochondrial); malate dehydrogenase, mitochondrial; mitochondrial malate dehydrogenase 2, NAD; MDH; Mor1; Mdh-2; Mor-1
• Gene ID: 17448
• mRNA Refseq: NM_008617
• Protein Refseq: NP_032643
• UniProt ID: P08249