Recombinant Mouse Med25 Protein, Myc/DDK-tagged

• Cat.No.: Med25-4017M
• Product Overview: Purified recombinant protein of mouse full-length mediator complex subunit 25 (Med25), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for RARA/RXRA-mediated transcription.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 78.1 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Med25 mediator complex subunit 25 [ Mus musculus (house mouse) ]
• Official Symbol: Med25
• Synonyms: MED25; mediator of RNA polymerase II transcription, subunit 25 homolog (yeast); mediator of RNA polymerase II transcription subunit 25; mMED25; mediator complex subunit 25; mediator of RNA polymerase II transcription, subunit 9 homolog; ESTM2; X83308; 2610034E13Rik; 2610529E18Rik
• Gene ID: 75613
• mRNA Refseq: NM_029365
• Protein Refseq: NP_083641
• UniProt ID: Q8VCB2