Recombinant Mouse Mfn2 Protein, Myc/DDK-tagged

• Cat.No.: Mfn2-4058M
• Product Overview: Purified recombinant protein of mouse full-length mitofusin 2 (Mfn2), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion. Mitochondria are highly dynamic organelles, and their morphology is determined by the equilibrium between mitochondrial fusion and fission events. Overexpression induces the formation of mitochondrial networks. Membrane clustering requires GTPase activity and may involve a major rearrangement of the coiled coil domains. Plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Plays an important role in the regulation of vascular smooth muscle cell proliferation. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). Is required for PRKN recruitment to dysfunctional mitochondria. Involved in the control of unfolded protein response (UPR) upon ER stress including activation of apoptosis and autophagy during ER stress. Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions.
• Molecular Mass: 86.2 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Mfn2 mitofusin 2 [ Mus musculus (house mouse) ]
• Official Symbol: Mfn2
• Synonyms: MFN2; mitofusin 2; mitofusin-2; HSG protein; transmembrane GTPase MFN2; hypertension related protein 1; hypertension-related protein 1; mitochondrial assembly regulatory factor; Fzo; D630023P19Rik
• Gene ID: 170731
• mRNA Refseq: NM_133201
• Protein Refseq: NP_573464
• UniProt ID: Q80U63