Recombinant Mouse Mid1 Protein, Myc/DDK-tagged

• Cat.No.: Mid1-4069M
• Product Overview: Purified recombinant protein of mouse full-length midline 1 (cDNA clone MGC:60591 IMAGE:30058736), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Has E3 ubiquitin ligase activity towards IGBP1, promoting its monoubiquitination, which results in deprotection of the catalytic subunit of protein phosphatase PP2A, and its subsequent degradation by polyubiquitination.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 50.1 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Mid1 midline 1 [ Mus musculus (house mouse) ]
• Official Symbol: Mid1
• Synonyms: Mid1; midline 1; Fxy; Trim; 61B3-; 61B3-R; Trim18; DXHXS114; DXHXS1141; E3 ubiquitin-protein ligase Midline-1; RING finger protein Midline-1; RING-type E3 ubiquitin transferase Midline-1; midin; tripartite motif-containing protein 18; EC 2.3.2.27
• Gene ID: 17318
• mRNA Refseq: NM_010797
• Protein Refseq: NP_034927
• UniProt ID: O70583