Recombinant Mouse Nanos2 Protein, Myc/DDK-tagged

• Cat.No.: Nanos2-4286M
• Product Overview: Purified recombinant protein of mouse full-length nanos C2HC-type zinc finger 2 (Nanos2), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Plays a key role in the sexual differentiation of germ cells by promoting the male fate but suppressing the female fate. Represses the female fate pathways by suppressing meiosis, which in turn results in the promotion of the male fate. Maintains the suppression of meiosis by preventing STRA8 expression, which is required for premeiotic DNA replication, after CYP26B1 is decreased. Regulates the localization of the CCR4-NOT deadenylation complex to P-bodies and plays a role in recruiting the complex to trigger the degradation of mRNAs involved in meiosis. Required for the maintenance of the spermatogonial stem cell population. Not essential for the assembly of P-bodies but is required for the maintenance of their normal state.
• Molecular Mass: 16 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Nanos2 nanos C2HC-type zinc finger 2 [ Mus musculus (house mouse) ]
• Official Symbol: Nanos2
• Synonyms: NANOS2; nanos homolog 2 (Drosophila); nanos homolog 2; NOS-2; nos2
• Gene ID: 378430
• mRNA Refseq: NM_194064
• Protein Refseq: NP_918953
• UniProt ID: P60322