Recombinant Mouse Nit2 Protein, Myc/DDK-tagged

• Cat.No.: Nit2-4420M
• Product Overview: Purified recombinant protein of mouse full-length nitrilase family, member 2 (Nit2), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Has omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting 2-oxoglutaramate and 2-oxosuccinamate to biologically useful 2-oxoglutarate and oxaloacetate, respectively. Can also hydrolyze gamma-monomethyl-alpha-ketoglutarate in vitro.
• Molecular Mass: 30.5 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Nit2 nitrilase family, member 2 [ Mus musculus (house mouse) ]
• Official Symbol: Nit2
• Synonyms: NIT2; nitrilase family, member 2; omega-amidase NIT2; Nit protein 2; nitrilase homolog 2; D16Ertd502e; 1190017B19Rik
• Gene ID: 52633
• mRNA Refseq: NM_023175
• Protein Refseq: NP_075664
• UniProt ID: Q9JHW2