Recombinant Mouse Nploc4 Protein, Myc/DDK-tagged

• Cat.No.: Nploc4-4473M
• Product Overview: Purified recombinant protein of mouse full-length NPL4 homolog, ubiquitin recognition factor (Nploc4), transcript variant B, with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: The ternary complex containing UFD1, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. Acts as a negative regulator of type I interferon production via the complex formed with VCP and UFD1, which binds to DDX58/RIG-I and recruits RNF125 to promote ubiquitination and degradation of DDX58/RIG-I.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 64.4 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Nploc4 NPL4 homolog, ubiquitin recognition factor [ Mus musculus (house mouse) ]
• Official Symbol: Nploc4
• Synonyms: NPLOC4; nuclear protein localization 4 homolog (S. cerevisiae); nuclear protein localization protein 4 homolog; Npl4; AK129375; mKIAA1499; RP23-82I5.6; KIAA1499
• Gene ID: 217365
• mRNA Refseq: NM_199469
• Protein Refseq: NP_955763
• UniProt ID: P60670