Recombinant Mouse Nudt5 Protein, Myc/DDK-tagged

• Cat.No.: Nudt5-4543M
• Product Overview: Purified recombinant protein of mouse full-length nudix (nucleoside diphosphate linked moiety X)-type motif 5 (Nudt5), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Enzyme that can either act as an ADP-sugar pyrophosphatase in absence of diphosphate or catalyze the synthesis of ATP in presence of diphosphate. In absence of diphosphate, hydrolyzes with similar activities various modified nucleoside diphosphates such as ADP-ribose, ADP-mannose, ADP-glucose, 8-oxo-GDP and 8-oxo-dGDP. Can also hydrolyze other nucleotide sugars with low activity. In presence of diphosphate, mediates the synthesis of ATP in the nucleus by catalyzing the conversion of ADP-ribose to ATP and ribose 5-phosphate. Nuclear ATP synthesis takes place when dephosphorylated at Thr-44. Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming. Does not play a role in U8 snoRNA decapping activity. Binds U8 snoRNA.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 24 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Nudt5 nudix (nucleoside diphosphate linked moiety X)-type motif 5 [ Mus musculus (house mouse) ]
• Official Symbol: Nudt5
• Synonyms: NUDT5; nudix (nucleoside diphosphate linked moiety X)-type motif 5; ADP-sugar pyrophosphatase; nudix motif 5; nucleoside diphosphate-linked moiety X motif 5
• Gene ID: 53893
• mRNA Refseq: NM_016918
• Protein Refseq: NP_058614
• UniProt ID: Q9JKX6