Recombinant Mouse Nup50 Protein, Myc/DDK-tagged

• Cat.No.: Nup50-4555M
• Product Overview: Purified recombinant protein of mouse full-length nucleoporin 50 (Nup50), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Component of the nuclear pore complex that has a direct role in nuclear protein import. Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin-alpha:beta-cargo complex and importin recycling. Interacts with regulatory proteins of cell cycle progression including CDKN1B. This interaction is required for correct intracellular transport and degradation of CDKN1B.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 49.5 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Nup50 nucleoporin 50 [ Mus musculus (house mouse) ]
• Official Symbol: Nup50
• Synonyms: NUP50; nucleoporin 50; nuclear pore complex protein Nup50; nucleoprotein 50; nucleoporin Nup50; 50 kDa nucleoporin; nuclear pore associated protein 60 kDa; Npap60; AI413123; 1700030K07Rik
• Gene ID: 18141
• mRNA Refseq: NM_016714
• Protein Refseq: NP_057923
• UniProt ID: Q9JIH2