Recombinant Mouse Olfm4, His-tagged

• Cat.No.: Olfm4-9170M
• Product Overview: Recombinant Mouse Olfm4 protein, fused to His-tag, was expressed in HEK293F cells and purified by His-tag affinity columns.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 538 amino acids
• Molecular Mass: The theoretical Molecular Weight of the recombinant protein (approximate): 61 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of recombinant mouse OLFM4 is approximately 65～70 kDa due to different glycosylation.
• Publications:
  The olfactomedin-4 positive neutrophil has a role in murine intestinal ischemia/reperfusion injury (2019)

Gene Information

• Gene Name: Olfm4 olfactomedin 4 [ Mus musculus ]
• Official Symbol: Olfm4
• Synonyms: OLFM4; olfactomedin 4; GC1; OlfD; pPD4; GW112; Gm296; Gm913
• Gene ID: 239192

Citation

Olfactomedin 4 deletion induces colon adenocarcinoma in Apc Min/+ mice

Journal: Oncogene    PubMed ID: 26973250    Data: 2016/10/6

Authors: W Liu, S-H Hong, G P Rodgers

Article Snippet:Human OLFM4 protein was purchased from Sino Biological Inc. (Beijing, China).Human OLFM4 protein was purchased from Sino Biological Inc. (Beijing, China).. Mouse Olfm4 protein was custom-made by Creative BioMart (New York, NY, USA).. OLFM4 antibody was purchased from Sino Biological Inc. or Abcam (Cambridge, MA, USA).OLFM4 antibody was purchased from Sino Biological Inc. or Abcam (Cambridge, MA, USA).

Olfm4 deletion induces large polyps in the small intestine and adenocarcinoma formation in the distal colon of Apc +/? mice. ( a ) Polyp formation (arrow) in the small intestine of Apc Min/+ , Apc +/? Olfm4 +/? and Apc +/? Olfm4 ?/? ( Apc Olfm4 double-mutant) mice. ( b ) Adenocarcinoma formation in the distal colon of Apc +/? Olfm4 +/? and Apc Olfm4 double-mutant mice. Three representative tumors in mice of each genotype are presented. Hematoxylin and eosin (HE) histology staining is shown below each corresponding tumor. Scale bar, 50 μm. ( c ) Mean (±s.d.) small intestinal polyp number and size, and colon-tumor incidence in Apc Min/+ ( n =20), Apc +/? Olfm4 +/? ( n =23) and Apc Olfm4 double-mutant mice ( n =22) mice.

Olfm4 deletion enhances Wnt/β-catenin signaling in Apc Olfm4 double-mutant mice. ( a–h ) β-Catenin immunohistochemistry staining was performed in the distal colon of Apc +/+ Olfm4 +/+ (WT) ( a ), Apc +/? Olfm4 +/+ ( b ), Apc +/+ Olfm4 ?/? ( c ), Apc +/? Olfm4 +/? ( d ) and Apc +/? Olfm4 ?/? ( e and g ) mice. Hematoxylin and eosin (HE) staining in ( f and h ) was performed in mice corresponding to ( e and g ), respectively. Scale bar, 50 μm. ( i ) A heatmap from cDNA microarray analysis shows Wnt pathway-related or target gene-expression level in the distal colon of mice with different genotypes. N, tumor-adjacent normal tissue; T, tumor. ( j ) Mean (±s.d.) ( n =3) gene-expression levels for six representative Wnt target genes were confirmed by qRT–PCR. See also and .

OLFM4 decreases β-catenin levels. ( a–k ) Western blots were performed in 293T cells ( a–i ), SW480 cells ( j ) and freshly isolated mouse colon crypts ( k ) after human OLFM4 treatment (1 μg/ml) ( a–j ) and mouse Olfm4 treatment (1 μg/ml) ( k ). β-Actin, total cadherin, Hsp90 and SP1 were used as loading controls in total cell lysate, membrane, cytoplasm and nucleus cell preparations, respectively. ( a ) β-Catenin levels in total cell lysate, membrane, cytoplasm and nucleus at different time points of OLFM4 treatment. ( b ) Endogenous and Wnt3a (1 μg/ml)-induced β-catenin levels in the cytoplasm and nucleus of cells pretreated with and without OLFM4 for 30 min. ( c ) Endogenous and Wnt3a (1 μg/ml)-induced β-catenin levels in the cytoplasm of cells transfected with control (vector) or OLFM4 expression plasmid. ( d ) β-Catenin phosphorylation at different time points of OLFM4 treatment. ( e and f ) Akt (T308 or Ser473) phosphorylation at different time points of OLFM4 treatment. ( g and h ) β-Catenin levels in the cytoplasm of cells pretreated with and without either LiCl (20 m m ) or MG132 (10 μ m ) before OLFM4 treatment for 30 min. ( i ) β-Catenin levels in total cell lysates of cells transfected with Flag-tagged WT or mutant (S33Y or S31/37T41S45) β-catenin expression plasmids and then treated with or without OLFM4 for 30 min. ( j ) Total and active β-catenin levels in the cytoplasm or nucleus of SW480 colon-cancer cells at different time points of Olfm4 treatment. ( k ) β-Catenin levels in the cytoplasm and nucleus of freshly isolated mouse colon crypts at different time points of Olfm4 (or PBS control buffer) treatment. ( l ) Olfm4 and β-catenin levels in the cytoplasm of Ls174T cells infected with lentiviral control shRNA and Olfm4 shRNA.