Recombinant Mouse Pdcd1lg2 Protein, hFc-tagged, Alexa Fluor 488 conjugated

• Cat.No.: Pdcd1lg2-7000MAF488
• Product Overview: Alexa Fluor 488 conjugated recombinant Mouse Pdcd1lg2 (Leu20-Pro219) protein was fused to human IgG1 Fc tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Species: Mouse
• Source: HEK293
• Tag: Fc
• Protein Length: Leu20-Arg219
• Description: Programmed cell death 1 ligand 2 (PD-L2 or PDCD1 ligand 2) is also known as Butyrophilin B7-DC, CD antigen CD273, which belongs to the immunoglobulin superfamily or BTN/MOG family. The expression of PD-L2 is up-regulated by IFNG/IFN-gamma stimulation in monocytes and induced on dendritic cells grown from peripheral blood mononuclear cells with CSF2 and IL-4. PD-L2 Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. PD-L2 interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.
• Form: Lyophilized
• Molecular Mass: The protein has a calculated MW of 49.2 kDa. As a result of glycosylation, the protein migrates as 60-70 kDa under reducing (R) condition, and 120-140 kDa under non-reducing (NR) condition (SDS-PAGE).
• N-terminal Sequence Analysis: Leu 20
• Endotoxin: < 1.0 EU/ μg by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 488 via amines
  Excitation Wavelength: 488 nm
  Emission Wavelength: 515­-545 nm
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
• Conjugation: Alexa Fluor 488

Gene Information

• Gene Name: Pdcd1lg2
• Official Symbol: Pdcd1lg2
• Synonyms: PDCD1LG2; programmed cell death 1 ligand 2; PD-1 ligand 2; PDCD1 ligand 2; butyrophilin B7-DC; butyrophilin-like protein; programmed death ligand 2; Btdc; B7-DC; PD-L2; F730015O22Rik; MGC124039; MGC124040
• Gene ID: 58205
• mRNA Refseq: NM_021396
• Protein Refseq: NP_067371
• UniProt ID: Q9WUL5