Recombinant Mouse Pdcd1lg2 Protein, His-tagged, Alexa Fluor 647 conjugated

• Cat.No.: Pdcd1lg2-7001MAF647
• Product Overview: Alexa Fluor 647 conjugated recombinant Mouse Pdcd1lg2 (Leu20-Pro219) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Description: Programmed cell death 1 ligand 2 (PD-L2 or PDCD1 ligand 2) is also known as Butyrophilin B7-DC, CD antigen CD273, which belongs to the immunoglobulin superfamily or BTN/MOG family. The expression of PD-L2 is up-regulated by IFNG/IFN-gamma stimulation in monocytes and induced on dendritic cells grown from peripheral blood mononuclear cells with CSF2 and IL-4. PD-L2 Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. PD-L2 interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.
• Source: HEK293
• Species: Mouse
• Tag: His
• Form: Lyophilized
• Molecular Mass: The protein has a calculated MW of 24.4 kDa. The protein migrates as 37-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Leu20-Arg219
• N-terminal Sequence Analysis: Leu 20
• Endotoxin: < 1.0 EU/ μg by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 647 via amines
  Excitation = 650 nm
  Emission = 668 nm
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: Pdcd1lg2
• Official Symbol: Pdcd1lg2
• Synonyms: PDCD1LG2; programmed cell death 1 ligand 2; PD-1 ligand 2; PDCD1 ligand 2; butyrophilin B7-DC; butyrophilin-like protein; programmed death ligand 2; Btdc; B7-DC; PD-L2; F730015O22Rik; MGC124039; MGC124040
• Gene ID: 58205
• mRNA Refseq: NM_021396
• Protein Refseq: NP_067371
• UniProt ID: Q9WUL5