Recombinant Mouse Plekhf1 Protein, Myc/DDK-tagged

• Cat.No.: Plekhf1-4928M
• Product Overview: Purified recombinant protein of mouse full-length pleckstrin homology domain containing, family F (with FYVE domain) member 1 (Plekhf1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: May induce apoptosis through the lysosomal-mitochondrial pathway. Translocates to the lysosome initiating the permeabilization of lysosomal membrane (LMP) and resulting in the release of CTSD and CTSL to the cytoplasm. Triggers the caspase-independent apoptosis by altering mitochondrial membrane permeabilization (MMP) resulting in the release of PDCD8.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 31.2 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Plekhf1 pleckstrin homology domain containing, family F (with FYVE domain) member 1 [ Mus musculus (house mouse) ]
• Official Symbol: Plekhf1
• Synonyms: PLEKHF1; pleckstrin homology domain containing, family F (with FYVE domain) member 1; pleckstrin homology domain-containing family F member 1; PH domain-containing family F member 1; lysosome-associated apoptosis-inducing protein containing PH and FYVE domains; APPD; LAPF; PHAFIN1; ZFYVE15; 1810013P09Rik
• Gene ID: 72287
• mRNA Refseq: NM_024413
• Protein Refseq: NP_077724
• UniProt ID: Q3TB82