Recombinant Mouse Poli Protein, Myc/DDK-tagged

• Cat.No.: Poli-4990M
• Product Overview: Purified recombinant protein of mouse full-length polymerase (DNA directed), iota (Poli), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.
• Molecular Mass: 75.1 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Poli polymerase (DNA directed), iota [ Mus musculus (house mouse) ]
• Official Symbol: Poli
• Synonyms: POLI; polymerase (DNA directed), iota; DNA polymerase iota; RAD30 homolog B; Rad30b
• Gene ID: 26447
• mRNA Refseq: NM_001136090
• Protein Refseq: NP_001129562
• UniProt ID: Q6R3M4