Recombinant Mouse Prpf19 Protein, Myc/DDK-tagged

• Cat.No.: Prpf19-5145M
• Product Overview: Purified recombinant protein of mouse full-length pre-mRNA processing factor 19 (Prpf19), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Ubiquitin-protein ligase which is a core component of several complexes mainly involved in pre-mRNA splicing and DNA repair. Required for pre-mRNA splicing as component of the spliceosome. Core component of the PRP19C/Prp19 complex/NTC/Nineteen complex which is part of the spliceosome and participates in its assembly, its remodeling and is required for its activity. During assembly of the spliceosome, mediates 'Lys-63'-linked polyubiquitination of the U4 spliceosomal protein PRPF3. Ubiquitination of PRPF3 allows its recognition by the U5 component PRPF8 and stabilizes the U4/U5/U6 tri-snRNP spliceosomal complex. Recruited to RNA polymerase II C-terminal domain (CTD) and the pre-mRNA, it may also couple the transcriptional and spliceosomal machineries. The XAB2 complex, which contains PRPF19, is also involved in pre-mRNA splicing, transcription and transcription-coupled repair. Beside its role in pre-mRNA splicing PRPF19, as part of the PRP19-CDC5L complex, plays a role in the DNA damage response/DDR. It is recruited to the sites of DNA damage by the RPA complex where PRPF19 directly ubiquitinates RPA1 and RPA2. 'Lys-63'-linked polyubiquitination of the RPA complex allows the recruitment of the ATR-ATRIP complex and the activation of ATR, a master regulator of the DNA damage response. May also play a role in DNA double-strand break (DSB) repair by recruiting the repair factor SETMAR to altered DNA. As part of the PSO4 complex may also be involved in the DNA interstrand cross-links/ICLs repair process. In addition, may also mediate 'Lys-48'-linked polyubiquitination of substrates and play a role in proteasomal degradation May play a role in the biogenesis of lipid droplets May play a role in neural differentiation possibly through its function as part of the spliceosome.
• Molecular Mass: 55.7 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Prpf19 pre-mRNA processing factor 19 [ Mus musculus (house mouse) ]
• Official Symbol: Prpf19
• Synonyms: PRPF19; PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae); pre-mRNA-processing factor 19; prp19 beta; PRP19/PSO4 homolog; senescence evasion factor; nuclear matrix protein 200; nuclear matrix protein SNEV; PSO4; Snev; Prp19; NMP200; AA617263; AL024362; D19Wsu55e
• Gene ID: 28000
• mRNA Refseq: NM_134129
• Protein Refseq: NP_598890
• UniProt ID: Q99KP6