Recombinant Mouse Rab9 Protein, Myc/DDK-tagged

• Cat.No.: Rab9-5334M
• Product Overview: Purified recombinant protein of mouse full-length RAB9, member RAS oncogene family (Rab9), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. RAD9A possesses 3'->5' double stranded DNA exonuclease activity.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 22.9 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Rab9 RAB9, member RAS oncogene family [ Mus musculus (house mouse) ]
• Official Symbol: Rab9
• Synonyms: Rab9; RAB9, member RAS oncogene family; Rab9a; AI195561; Sid6061p; 2410064E05Rik; ras-related protein Rab-9A; SID 99; rab-9A
• Gene ID: 56382
• mRNA Refseq: NM_019773
• Protein Refseq: NP_062747
• UniProt ID: Q9R0M6