Recombinant Mouse Rec8 Protein, Myc/DDK-tagged

• Cat.No.: Rec8-5447M
• Product Overview: Purified recombinant protein of mouse full-length REC8 meiotic recombination protein (Rec8), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 67.4 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Rec8 REC8 meiotic recombination protein [ Mus musculus (house mouse) ]
• Official Symbol: Rec8
• Synonyms: REC8; REC8 homolog (yeast); meiotic recombination protein REC8 homolog; REC8-like 1; cohesin Rec8p; meiotic cohesion Rec8; mrec; Rec8L1
• Gene ID: 56739
• mRNA Refseq: NM_020002
• Protein Refseq: NP_064386
• UniProt ID: Q8C5S7