Recombinant Mouse Rffl Protein, Myc/DDK-tagged

• Cat.No.: Rffl-5477M
• Product Overview: Purified recombinant protein of mouse full-length ring finger and FYVE like domain containing protein (Rffl), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: E3 ubiquitin-protein ligase that regulates several biological processes through the ubiquitin-mediated proteasomal degradation of various target proteins. Mediates 'Lys-48'-linked polyubiquitination of PRR5L and its subsequent proteasomal degradation thereby indirectly regulating cell migration through the mTORC2 complex. Also ubiquitinates the caspases CASP8 and CASP10, promoting their proteasomal degradation, to negatively regulate apoptosis downstream of death domain receptors. Also negatively regulates the tumor necrosis factor-mediated signaling pathway through targeting of RIPK1 to ubiquitin-mediated proteasomal degradation. Negatively regulates p53/TP53 through its direct ubiquitination and targeting to proteasomal degradation. Indirectly, may also negatively regulate p53/TP53 through ubiquitination and degradation of SFN. May also play a role in endocytic recycling.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 37.4 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Rffl ring finger and FYVE like domain containing protein [ Mus musculus (house mouse) ]
• Official Symbol: Rffl
• Synonyms: RFFL; ring finger and FYVE like domain containing protein; E3 ubiquitin-protein ligase rififylin; fring; rififylin; RING finger and FYVE-like domain-containing protein 1; Carp2; BG080975; 1700051E09Rik; 4930516L10Rik; MGC144763
• Gene ID: 67338
• mRNA Refseq: NM_026097
• Protein Refseq: NP_080373
• UniProt ID: Q6ZQM0