Recombinant Mouse RGMB Protein (Met1-Cys415), His-tagged RGMB-889M

Recombinant Mouse RGMB Protein (Met1-Cys415), His-tagged

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Recombinant Mouse RGMB Protein (Met1-Cys415), His-tagged

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Cat.No. : RGMB-889M
Product Overview : Recombinant Mouse RGMB Protein (NP_848730.2) (Met1-Cys415) was produced by HEK293 Cells expression system. This protein was expressed with a polyhistidine tag at the C-terminus.
Description : RGMB was knocked down in breast cancer cells by way of an anti-RGMB ribozyme transgene. Knockdown of RGMB resulted in enhanced capacities of proliferation, adhesion, and migration in breast cancer cells. Further investigations demonstrated RGMB knockdown resulted in a reduced expression and activity of Caspase-3, accompanied with better survival in RGMB knockdown cells under serum starvation, which might be induced by its repression on MAPK JNK pathway. That RGMB may act as a negative regulator in breast cancer through BMP signaling.
Source : HEK293
Species : Mouse
Tag : His
Predicted N Terminal : Gly 49
Form : Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Molecular Mass : The recombinant mouse RGMB consists of 377 amino acids and predicts a molecular mass of 41.6 kDa.
Protein length : Met1-Cys415
Endotoxin : < 1.0 EU per μg protein as determined by the LAL method.
Purity : > (26.4+56.4+14.9)% as determined by SDS-PAGE.
Stability : Samples are stable for up to twelve months from date of receipt at -70 centigrade.
Storage : Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Shipping : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Gene Name : Rgmb RGM domain family, member B [ Mus musculus ]
Official Symbol : RGMB
Synonyms : RGMB; RGM domain family, member B; DRAGON; 1110059F19Rik;
Gene ID : 68799
mRNA Refseq : NM_178615
Protein Refseq : NP_848730
UniProt ID : Q7TQ33

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