Recombinant Mouse Rnaseh1 Protein, Myc/DDK-tagged

• Cat.No.: Rnaseh1-5536M
• Product Overview: Purified recombinant protein of mouse full-length ribonuclease H1 (Rnaseh1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: This gene encodes an endonuclease that specifically degrades the RNA of RNA-DNA hybrids and is necessary for DNA replication and repair. This enzyme is present in both mitochondria and nuclei, which are resulted from translation of a single mRNA with two in-frame initiation start codons. The use of the first start codon produces the mitochondrial isoform and the use of the second start codon produces the nuclear isoform. The production of the mitochondrial isoform is modulated by an upstream open reading frame (uORF) which encodes 7aa in mouse. An alternately spliced transcript variant has been found which is a candidate for nonsense-mediated mRNA decay (NMD).
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 31.8 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Rnaseh1 ribonuclease H1 [ Mus musculus (house mouse) ]
• Official Symbol: Rnaseh1
• Synonyms: RNASEH1; ribonuclease H1; RNase H1
• Gene ID: 19819
• mRNA Refseq: NM_011275
• Protein Refseq: NP_035405
• UniProt ID: E9QLN8