Recombinant Mouse Rpn2 Protein, Myc/DDK-tagged

• Cat.No.: Rpn2-5596M
• Product Overview: Purified recombinant protein of mouse full-length ribophorin II (Rpn2), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc3Man9GlcNAc2 in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.
• Molecular Mass: 69.1 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Rpn2 ribophorin II [ Mus musculus (house mouse) ]
• Official Symbol: Rpn2
• Synonyms: RPN2; ribophorin II; dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2; RPN-II; ribophorin-2; dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa subunit; Rpn-2; AV261018; 1300012C06Rik
• Gene ID: 20014
• mRNA Refseq: NM_019642
• Protein Refseq: NP_062616
• UniProt ID: Q9DBG6