Recombinant Mouse Rspo1 Protein, Fc-tagged
Cat.No. : | Rspo1-054M |
Product Overview : | Recombinant mouse RSPO1 mFc Chimera (rmRSPO1-mFc), Met1-Asn 932 (Accession # P08069) was produced in human HEK293 cells. |
- Specification
- Gene Information
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Description : | Mouse R-Spondin 1 ( Roof plate-specific Spondin 1 ), also known as RSPO1 and CRISTIN3, FLJ40906 or RP11-566C13.1, Which is a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain, which belongs to the R-Spondin family. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. R-Spondin1 activity critically depends on the presence of canonical Wnt ligands and LRP6. R-Spondin1 does not directly activate LRP6, it interferes with DKK1 / Kremen-mediated internalization of LRP6 through an interaction with Kremen, resulting in increased LRP6 levels on the cell surface. Mouse R-Spondin1 shares 89%, 87%, 92%, 91%, 91%, and 89% aa identity with human R-Spondin1. Defects in RSPO1 are the cause of palmoplantar keratoderma with squamous cell carcinoma of skin and sex reversal (PKKSCC). This recessive syndrome is characterized by XX (female to male) SRY-independent sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin. |
Source : | HEK293 cells |
Species : | Mouse |
Tag : | Fc |
Molecular Mass : | rmRSPO1-mFc was fused with the mouse Fc region of at the C-terminus, the reduced monomer has a calculated molecular mass of 58.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmRSPO1-mFc monomer is approximately 67-75 kDa due to the glycosylation. |
Endotoxin : | Less than 1.0 EU per 1 µg of the rmRSPO1-mFc by the LAL method. |
Purity : | >95% as determined by SDS-PAGE. All lots are greater than 95% pure. |
Notes : | Research Use Only. Not for use in diagnostic or therapeutic procedures. |
Storage : | Store at -20 centigrade in lyophilized state after receipt. For long term storage, upon reconstitution rmRSPO1-mFc should be aliquot and store at -20 centigrade or -8 centigrade centigrade. Avoid repeated freeze-thaw cycles. |
Storage Buffer : | Bulk protein in a 0.22 µm filtered solution of PBS, pH 7.4 and delivered as liquid formulation or lyophilized powder. Normally 5-8% trehalose and mannitol are added as protectants before lyophilization. |
Gene Name : | Rspo1 R-spondin homolog (Xenopus laevis) [ Mus musculus ] |
Official Symbol : | Rspo1 |
Synonyms : | RSPO1; R-spondin homolog (Xenopus laevis); R-spondin-1; cristin-3; mCristin-3; roof plate-specific spondin-1; thrombospondin type 1 domain containing gene Rspondin; cysteine-rich and single thrombospondin domain-containing protein 3; Rspondin; R-spondin; |
Gene ID : | 192199 |
mRNA Refseq : | NM_138683 |
Protein Refseq : | NP_619624 |
Products Types
◆ Recombinant Protein | ||
RSPO1-422H | Recombinant Human RSPO1 Protein, His-tagged | +Inquiry |
RSPO1-132H | Active Recombinant Human RSPO1 Protein (Ser21-Ala263), N-His-SUMO tagged, Animal-free, Carrier-free | +Inquiry |
Rspo1-8672M | Active Recombinant Mouse Rspo1 protein(Met1-Gln265), His-tagged | +Inquiry |
RSPO1-421H | Recombinant Human RSPO1 Protein, His-tagged | +Inquiry |
RSPO1-28H | Active Recombinant Human RSPO1 Protein, Pre-aliquoted | +Inquiry |
◆ Lysates | ||
RSPO1-001MCL | Recombinant Mouse RSPO1 cell lysate | +Inquiry |
RSPO1-1918HCL | Recombinant Human RSPO1 cell lysate | +Inquiry |
RSPO1-001HCL | Recombinant Human RSPO1 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionRSPO1 stands out for its potential therapeutic use in Regenerative Medicine due to its mitogenic activity in stem cells.
Characterization methods included Western blotting, mass spectrometry, and in vitro (C2C12 cells) and in vivo (BALB/c mice) biological activity assays, confirming structural integrity and biological efficacy.
RSPOs regulate various tissue-specific processes, including bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells, intestinal stem cells, and cancer progression.
The purification strategy for rhRSPO1 involved two sequential chromatographic steps: heparin affinity chromatography followed by molecular exclusion chromatography.
RSPO1 induces the Wnt pathway by stabilizing the Wnt receptor complex, leading to enhanced Wnt signaling and influencing cellular processes.
rhRSPO1 was generated using the HEK293 cell line, and the coding sequence was synthesized and subcloned into a mammalian cell expression vector.
R-Spondin proteins, including RSPO1, play crucial roles in cell proliferation, differentiation, and death by inducing the Wnt pathway.
Customer Reviews (3)
Write a reviewRemarkable changes in cell behavior were attributed to the potent effects of the product.
The product's respected reputation in the scientific community was a testament to its quality and effectiveness.
Dependable protein-protein interaction outcomes were achieved due to the product's pure composition.
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