Recombinant Mouse RSPO1 protein, Flag-tagged, For Organoid Culture

• Cat.No.: RSPO1-4343M
• Product Overview: Recombinant Mouse RSPO1 protein(NP_619624.2)(Ser21-Gln265) is expressed in HEK293 with a Flag tag at C-terminus.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Flag
• Protein Length: Ser21-Gln265
• Tag: C-Flag
• Form: Supplied as a 0.2 μm filtered solution of PBS, pH7.4.
• Bio-activity: Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The typical ED50 is 81.53ng/mL in the presence of 5 ng/mL Recombinant Human Wnt3a V3.
• Molecular Mass: 28.1 KDa
• Endotoxin: < 1 EU/μg as determined by LAL test.
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.