Recombinant Mouse SIRT1 protein, His-tagged

• Cat.No.: SIRT1-101M
• Product Overview: Recombinant Mouse SIRT1 protein (Glu236-Ala490), fused to His-tag at N-terminus, was expressed in E.coli.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: 255
• Description: This gene encodes a member of the sirtuin family of proteins, characterized by their deacetylase activity and proposed role in longevity. The encoded protein regulates gene expression in a wide range of cell and tissue types through its NAD+-dependent deacetylation of histones, transcription factors and transcriptional coactivators. Brain-specific overexpression of this gene has been shown to result in increased median lifespan. Viability of homozygous knockout mice for this gene varies with strain background. Homozygous knockout mice of strains that do not exhibit embryonic lethality are sterile and have a reduced lifespan. Alternative splicing results in multiple transcript variants.
• Form: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin 300.
• Molecular Mass: 30.0 kDa
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >97%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Sirt1
• Official Symbol: Sirt1
• Synonyms: Sir2; Sir2a; SIR2L1; AA673258; Sir2alpha
• Gene ID: 93759
• mRNA Refseq: NM_019812.3
• Protein Refseq: NP_062786.1
• UniProt ID: Q923E4