Recombinant Mouse Smo Protein, His-tagged

• Cat.No.: Smo-2084M
• Product Overview: Recombinant Mouse Smo Protein (Ala33-Ile238) with N-His tag was expressed in E. coli.

Specification

• Description: Predicted to enable patched binding activity. Involved in several processes, including animal organ development; regulation of animal organ morphogenesis; and regulation of gene expression. Acts upstream of or within several processes, including animal organ development; positive regulation of cell population proliferation; and regionalization. Located in 9+0 non-motile cilium; ciliary membrane; and cytoplasm. Is expressed in several structures, including 1st branchial arch; 4-cell stage embryo; alimentary system; central nervous system; and genitourinary system. Used to study medulloblastoma and pulmonary emphysema. Human ortholog(s) of this gene implicated in basal cell carcinoma; hepatocellular carcinoma; pancreatic cancer; and pancreatic ductal carcinoma. Orthologous to human SMO (smoothened, frizzled class receptor).
• Source: E. coli
• Species: Mouse
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 26.2 kDa
  Accurate Molecular Mass: 31 kDa
• Protein length: Ala33-Ile238
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: Smo smoothened homolog (Drosophila) [ Mus musculus (house mouse) ]
• Official Symbol: Smo
• Synonyms: SMO; smoothened homolog (Drosophila); smoothened homolog; bent body; bnb; Smoh; smoothened; E130215L21Rik
• Gene ID: 319757
• mRNA Refseq: NM_176996
• Protein Refseq: NP_795970
• UniProt ID: P56726