"Spock3" Related Products

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Recombinant Mouse Spock3 Protein, His-tagged

Cat.No.: Spock3-871M
Product Overview: Recombinant mouse Spock3 (NP_076178.1) (Met1-Ile436) was expressed with a polyhistidine tag at the C-terminus.
Source: Baculovirus-Insect Cells
Species: Mouse
Tag: His
Predicted N Terminal: Ala 23
Form: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4, 5%~8% trehalose and mannitol.
Bio-activity: Measured by its ability to inhibit active Cathepsin L cleavage of a fluorogenic peptide substrate Z-Leu-Arg-AMC. The IC50 value is< 15 nM.
Molecular Mass: The recombinant mouse Spock3 consists 425 amino acids and predicts a molecular mass of 48.3 kDa.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >90 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: Spock3 sparc/osteonectin, cwcv and kazal-like domains proteoglycan 3 [ Mus musculus ]
Official Symbol: Spock3
Synonyms: SPOCK3; sparc/osteonectin, cwcv and kazal-like domains proteoglycan 3; testican-3; testican 3; AI428471; mKIAA4039; 2900045C01Rik; KIAA4039;
Gene ID: 72902
mRNA Refseq: NM_001252620
Protein Refseq: NP_001239549
MIM:
UniProt ID:
Function: calcium ion binding; enzyme inhibitor activity; glycosaminoglycan binding; metalloendopeptidase inhibitor activity;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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