Recombinant Mouse Tcea3 Protein, Myc/DDK-tagged

• Cat.No.: Tcea3-6329M
• Product Overview: Purified recombinant protein of mouse full-length transcription elongation factor A (SII), 3 (Tcea3), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.
• Molecular Mass: 38.8 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Tcea3 transcription elongation factor A (SII), 3 [ Mus musculus (house mouse) ]
• Official Symbol: Tcea3
• Synonyms: TCEA3; transcription elongation factor A (SII), 3; transcription elongation factor A protein 3; transcription elongation factor TFIIS.h; tissue-specific transcription factor S-II; transcription elongation factor S-II protein 3; S-II; SII-K1
• Gene ID: 21401
• mRNA Refseq: NM_011542
• Protein Refseq: NP_035672
• UniProt ID: P23881