Active Recombinant Mouse Tnfsf9 protein, hFc-tagged, Low Endotoxin

• Cat.No.: Tnfsf9-133M
• Product Overview: Recombinant Mouse Tnfsf9 protein(NP_033430)(104-309 aa), fused with C-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Protein Length: 104-309 aa
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Mouse 4-1BB, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Mouse 4-1BB Ligand, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 49.7 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: Tnfsf9 tumor necrosis factor (ligand) superfamily, member 9 [ Mus musculus ]
• Official Symbol: Tnfsf9
• Synonyms: TNFSF9; tumor necrosis factor (ligand) superfamily, member 9; tumor necrosis factor ligand superfamily member 9; 4-1BB ligand; Ly63l; 4-1BBL; Cd137l; 4-1BB-L; AI848817
• Gene ID: 21950
• mRNA Refseq: NM_009404
• Protein Refseq: NP_033430