Recombinant Mouse Tpx2 Protein, Myc/DDK-tagged

• Cat.No.: Tpx2-6615M
• Product Overview: Purified recombinant protein of mouse full-length TPX2, microtubule-associated (Tpx2), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Spindle assembly factor required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation. TPX2 is inactivated upon binding to importin-alpha. At the onset of mitosis, GOLGA2 interacts with importin-alpha, liberating TPX2 from importin-alpha, allowing TPX2 to activates AURKA kinase and stimulates local microtubule nucleation.
• Molecular Mass: 85.9 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Tpx2 TPX2, microtubule-associated [ Mus musculus (house mouse) ]
• Official Symbol: Tpx2
• Synonyms: TPX2; TPX2, microtubule-associated protein homolog (Xenopus laevis); targeting protein for Xklp2; DIL2; p100; REPP86; 2610005B21Rik
• Gene ID: 72119
• mRNA Refseq: NM_001141978
• Protein Refseq: NP_001135450
• UniProt ID: A2APB8