Recombinant Mouse Ube2t Protein, Myc/DDK-tagged

• Cat.No.: Ube2t-6799M
• Product Overview: Purified recombinant protein of mouse full-length ubiquitin-conjugating enzyme E2T (Ube2t), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in mitomycin-C (MMC)-induced DNA repair: acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. Also mediates monoubiquitination of FANCL and FANCI. May contribute to ubiquitination and degradation of BRCA1. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 23 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Ube2t ubiquitin-conjugating enzyme E2T [ Mus musculus (house mouse) ]
• Official Symbol: Ube2t
• Synonyms: UBE2T; ubiquitin-conjugating enzyme E2T (putative); ubiquitin-conjugating enzyme E2 T; ubiquitin-protein ligase T; ubiquitin carrier protein T; C80607; 2700084L22Rik
• Gene ID: 67196
• mRNA Refseq: NM_026024
• Protein Refseq: NP_080300
• UniProt ID: Q9CQ37