Recombinant Mouse UBE3A Protein (542-870 aa), His-tagged

• Cat.No.: UBE3A-1986M
• Product Overview: Recombinant Mouse UBE3A Protein (542-870 aa) is produced by E. coli expression system. This protein is fused with a 6xHis tag at the N-terminal. Research Area: Epigenetics and Nuclear Signaling. Protein Description: Partial.

Specification

• Description: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B. Additionally, may function as a cellular quality control ubiquitin ligase by helping the degradation of the Cytoplasmic domain misfolded proteins. Finally, UBE3A also promotes its own degradation in vivo (By similarity). Plays an important role in the regulation of the circadian clock: involved in the ubiquitination of the core clock component ARNTL/BMAL1, leading to its proteasomal degradation (PubMed:24728990).
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 41.9 kDa
• Protein length: 542-870 aa
• AA Sequence: NPADLKKQLYVEFEGEQGVDEGGVS KEFFQLVVEEIFNPDIGMFTYDEAT KLFWFNPSSFETEGQFTLIGIVLGL AIYNNCILDVHFPMVVYRKLMGKKG TFRDLGDSHPVLYQSLKDLLEYEGS VEDDMMITFQISQTDLFGNPMMYDL KENGDKIPITNENRKEFVNLYSDYI LNKSVEKQFKAFRRGFHMVTNESPL KYLFRPEEIELLICGSRNLDFQALE ETTEYDGGYTRESVVIREFWEIVHS FTDEQKRLFLQFTTGTDRAPVGGLG KLKMIIAKNGPDTERLPTSHTCFNV LLLPEYSSKEKLKERLLKAITYAKG FGML
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with reconstitution instruction is sent along with the products.

Gene Information

• Gene Name: Ube3a ubiquitin protein ligase E3A [ Mus musculus ]
• Official Symbol: UBE3A
• Synonyms: UBE3A; Hpve6a; mKIAA4216; 4732496B02; 5830462N02Rik; A130086L21Rik; KIAA4216
• Gene ID: 22215
• mRNA Refseq: NM_001033962
• Protein Refseq: NP_001029134
• UniProt ID: O08759