Recombinant Mouse Vhl Protein, Myc/DDK-tagged

• Cat.No.: Vhl-6921M
• Product Overview: Purified recombinant protein of Mouse von Hippel-Lindau tumor suppressor (Vhl), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Involved in the ubiquitination and subsequent proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Seems to act as a target recruitment subunit in the E3 ubiquitin ligase complex and recruits hydroxylated hypoxia-inducible factor (HIF) under normoxic conditions. Involved in transcriptional repression through interaction with HIF1A, HIF1AN and histone deacetylases. Ubiquitinates, in an oxygen-responsive manner, ADRB2.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 20.8 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Vhl von Hippel-Lindau tumor suppressor [ Mus musculus (house mouse) ]
• Official Symbol: Vhl
• Synonyms: VHL; von Hippel-Lindau tumor suppressor; von Hippel-Lindau disease tumor suppressor; pVHL; von Hippel-Lindau syndrome homolog; von Hippel-Lindau syndrome protein homolog; Vhlh
• Gene ID: 22346
• mRNA Refseq: NM_009507
• Protein Refseq: NP_033533
• UniProt ID: P40338