Recombinant Mouse Vsir protein, His-tagged

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Recombinant Mouse Vsir protein, His-tagged

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Cat.No. : Vsir-717M
Product Overview : Recombinant Mouse Vsir (Phe33-Ala191) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).
Description : Platelet receptor Gi24, also known as B7-H5 and stress-induced secreted protein-1 (Sisp-1), is a protein that in humans is encoded by the C10orf54 gene, which contains 1 Ig-like (immunoglobulin-like) domain. As for C10orf54 gene, C10orf54 appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state on ESCs. Human C10orf54 undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30 kDa extracellular fragment plus a 25-30 kDa membrane-bound fragment.
Source : HEK293
Species : Mouse
Tag : His
Predicted N Terminal : Phe33
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 19.7kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : Phe33-Ala191
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of Vsir-717M :

Mouse B7-H5, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Gene Name : Vsir
Official Symbol : Vsir
Synonyms : Dies1; PD-1H; VISTA; 4632428N05Rik
Gene ID : 74048
mRNA Refseq : NM_028732.4
Protein Refseq : NP_083008.1
UniProt ID : Q9D659

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