Recombinant Mouse Vsir protein, His-tagged

• Cat.No.: Vsir-717M
• Product Overview: Recombinant Mouse Vsir (Phe33-Ala191) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Description: Platelet receptor Gi24, also known as B7-H5 and stress-induced secreted protein-1 (Sisp-1), is a protein that in humans is encoded by the C10orf54 gene, which contains 1 Ig-like (immunoglobulin-like) domain. As for C10orf54 gene, C10orf54 appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state on ESCs. Human C10orf54 undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30 kDa extracellular fragment plus a 25-30 kDa membrane-bound fragment.
• Source: HEK293
• Species: Mouse
• Tag: His
• Predicted N Terminal: Phe33
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 19.7kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Phe33-Ala191
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: Vsir
• Official Symbol: Vsir
• Synonyms: Dies1; PD-1H; VISTA; 4632428N05Rik
• Gene ID: 74048
• mRNA Refseq: NM_028732.4
• Protein Refseq: NP_083008.1
• UniProt ID: Q9D659