Recombinant Mouse Wee1 Protein, Myc/DDK-tagged

• Cat.No.: Wee1-6992M
• Product Overview: Purified recombinant protein of Mouse WEE 1 homolog 1 (S. pombe) (Wee1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 71.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Wee1 WEE 1 homolog 1 (S. pombe) [ Mus musculus (house mouse) ]
• Official Symbol: Wee1
• Synonyms: WEE1; WEE 1 homolog 1 (S. pombe); wee1-like protein kinase; wee1A kinase; WEE1 tyrosine kinase; Wee1A
• Gene ID: 22390
• mRNA Refseq: NM_009516
• Protein Refseq: NP_033542
• UniProt ID: P47810