Recombinant MPXV H3L protein, His-tagged

• Cat.No.: H3L-0407M
• Product Overview: Recombinant MPXV H3L protein, fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: MPXV
• Tag: His
• Form: Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Molecular Mass: The recombinant Monkeypox Virus (MPXV) Envelope protein H3 consists of 289 amino acids and predicts a molecular mass of 33.69 kDa. It migrates as an approximately 40.17 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.