Recombinant NiV(strain Bangladesh 2005) G protein, His-tagged

• Cat.No.: G-5743N
• Product Overview: Recombinant NiV(strain Bangladesh 2005) G protein(AAY43916.1) (Gln71-Thr602) was expressed in HEK293, with a polyhistidine tag at the C-terminus.

Specification

• Species: NiV
• Source: HEK293
• Tag: His
• Protein Length: 71-602 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Immobilized Recombinant Human Ephrin-B2 / EFNB2 Protein at 2 μg/mL (100 μL/well) can bind Recombinant Nipah virus Glycoprotein G Protein (ECD, His Tag), the EC50 is 16-48 ng/mL.
• Molecular Mass: The recombinant NIV Glycoprotein G consists of 543 amino acids and predicts a molecular mass of 60.97 kDa. It migrates as an approximately 65.1 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: ≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.