Recombinant Phleum pratense Phl p 5a Allergen, His tagged

• Cat.No.: Phl p 5a-045P
• Product Overview: The Phleum pratense major allergen Phl p 5 has been prepared as the recombinant mature allergen fused to a his-tag in its N-terminus.

Specification

• Species: Phleum pratense
• Source: E.coli
• Tag: His
• Description: Phl p 5a is a major allergen of grass pollen (Phleum pratense). It has been prepared as the recombinant mature allergen fused to a his-tag.
• Tag: N-His
• Molecular Mass: Determined by SDS-PAGE, the protein band is between molecular markers of 35 and 45 kDa, while relative molecular mass calculated from amino acid sequence is 35,224.5 Da.
• Purity: > 95% by observation on SDS-PAGE electrophoresis
• Applications: WB, DB, IE, DE, CLIA, LF
• Storage: Upon arrival, it should be aliquoted to avoid repeated freezing and thawing cycles and stored at -20 to -80 centigrade. In order to defrost the protein, maintain the aliquot at 25 centigrade without shaking to avoid aggregation.
• Storage Buffer: 20 mM phosphate buffer pH 7 and 0.15 M NaCl
• Concentration: 1.03 mg/mL
• Shipping: Protein is shipped with dry ice.
• Reference: 1. Julia Hecker et al. Generation and epitope analysis of human monoclonal antibody isotypes with specificity for the timothy grass major allergen Phl p 5a. 2011, Molecular Immunology 48:1236-1244.
  2. Gill SC, von Hippel PH. Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem. 1989 Nov 1;182(2):319-26.