Recombinant Pig Carboxypeptidase B

• Cat.No.: CPB-5P

Specification

• Description: Carboxypeptidase B, also well known as pancreatic procarboxy-peptidase B (PCPB), is a highly pancreas-specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitisand pancreatic graft rejection. As the prototype for those humanexopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lys residues with a preferencefor Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point.
• Species: Pig
• Form: Lyophilized or Liquid
• Molecular Mass: The deduced amino acid sequence of PCPB predicts a 416-amino acid preproenzyme consisting of a 15-aa signal peptide, a 95-aa activation peptide and a 307-aa mature chain.The secreted PCPB zymogen is converted to enzymatically active CPB by limited proteolysis by trypsin.
• Unit Definition: One Unit hydrolyzes one micromole of hippuryl-L-arginine per minute at 25°C, pH 7.65.
• Enzyme Unit: ≥70 units per mg protein.
• Storage: Store at -20 °C, for extended storage, conserve at -20 °C or -80 °C.
• Concentration: 1 -10 mg/mL
• Storage Buffer: In 100 mM sodium chloride
• Warning: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week.

Gene Information

• Gene Name: CPB1 carboxypeptidase B1 (tissue) [ Sus scrofa(pig) ]
• Official Symbol: CPB1
• Synonyms: CPB1; CPB
• Gene ID: 397341
• mRNA Refseq: NM_214169
• Protein Refseq: NP_999334
• UniProt ID: P09955
• Chromosome Location: 13; 13
• Pathway: Pancreatic secretion, organism-specific biosystem; Protein digestion and absorption, organism-specific biosystem
• Function: metallocarboxypeptidase activity; zinc ion binding