Species : |
Pig |
Tag : |
Non |
Description : |
Carboxypeptidase B, also well known as pancreatic procarboxy-peptidase B (PCPB), is a highly pancreas-specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitisand pancreatic graft rejection. As the prototype for those humanexopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lys residues with a preferencefor Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point. |
Form : |
Lyophilized or Liquid |
Molecular Mass : |
The deduced amino acid sequence of PCPB predicts a 416-amino acid preproenzyme consisting of a 15-aa signal peptide, a 95-aa activation peptide and a 307-aa mature chain.The secreted PCPB zymogen is converted to enzymatically active CPB by limited proteolysis by trypsin. |
Unit Definition : |
One Unit hydrolyzes one micromole of hippuryl-L-arginine per minute at 25°C, pH 7.65. |
Enzyme Unit : |
≥70 units per mg protein. |
Storage : |
Store at -20 °C, for extended storage, conserve at -20 °C or -80 °C. |
Concentration : |
1 -10 mg/mL |
Storage Buffer : |
In 100 mM sodium chloride |
Warning : |
Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. |