Active Recombinant Pig IFNA1 protein, His-GST-tagged

• Cat.No.: IFNA1-640P
• Product Overview: Active Recombinant Pig IFNA1 protein(P49879)(24~172 aa), fused with N-terminal His tag and GST tag, was expressed in E.coli.

Specification

• Species: Pig
• Source: E.coli
• Tag: GST&His
• Protein Length: 24-172 aa
• Form: Lyophilized from sterile PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Bio-activity: Interferon-alpha (IFNa), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits. IFN-α has both anti-viral and immunomodulatory activities on target cells. To test the effect of IFNa on cell apoptosis, A549 cells were seeded into triplicate wells of 96-well plates at a density of 4,000 cells/well and allowed to attach overnight, then the medium was replaced with various concentrations of recombinant pig IFNa diluted with 5% serum standard DMEM. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Apoptosis of A549 cells after incubation with IFNa for 72h observed by inverted microscope was shown. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant IFNa for 72h. It was obvious that IFNa significantly decreased cell viability of A549 cells. The ED50 of recombinant pig IFNa is 9.24 ug/ml.
• Molecular Mass: 47.0kDa
• Endotoxin: <1.0EU per 1μg (determined by the LAL method).
• Purity: > 95% as determined by SDS-PAGE.
• Storage: Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: IFNA1 interferon alpha-1 [ Sus scrofa ]
• Official Symbol: IFNA1
• Synonyms: IFNA1; interferon alpha-1; IFN-alpha-6; interferon alpha 1; interferon-alpha-6; IFN1@; IFN-ALPHA-1
• Gene ID: 397686
• mRNA Refseq: NM_214393
• Protein Refseq: NP_999558