Active Recombinant Pig IL8 protein, His-tagged

Cat.No. : IL8-601P
Product Overview : Active Recombinant Pig IL8 protein(P26894)(26~103 aa), fused with N-terminal His tag, was expressed in E.coli.
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Species : Pig
Source : E.coli
Tag : His
Protein Length : 26-103 aa
Form : Lyophilized from sterile PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Bio-activity : Interleukin 8 (IL8 or chemokine (C-X-C motif) ligand 8, CXCL8) is a chemokine produced by macrophages and other cell types such as epithelial cells, airway smooth muscle cells and endothelial cells.Thus, chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of IL8 on the human T-lymphocyte leukemia cell line Jurkat. Briefly, Jurkat cells were seeded into the upper chambers (100ul cell suspension,10^6 cells/ml in RPMI 1640 with FBS free) and recombinant pig IL8 (1ng/ml, 10ng/ml,100ng/ml and 1000ng/ml diluted separately in serum free RPMI 1640 ) was added in lower chamber with a polycarbonate filter(8um pore size) used to separate the two compartments. After incubation at 37°C with 5% CO2 for 1h, the filter was removed,then cells in low chamber were observed by inverted microscope at low magnification (×100) and the number of migrated cells were counted at high magnification(×400) randomly (five fields for each filter). Result shows IL8 is able to induce migration of Jurkat cells. The migrated Jurkat cells in low chamber at low magnification(×100) were shown. Five fields of each chamber were randomly chosen, and the migrated cells were counted at high magnification(×400). The optimum chemotaxis of IL8 occurs at 1~10ng/ml.
Molecular Mass : 13.1kDa
Endotoxin : <1.0EU per 1μg (determined by the LAL method).
Purity : > 97% as determined by SDS-PAGE.
Storage : Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
Reconstitution : Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Gene Name IL8 interleukin 8 [ Sus scrofa ]
Official Symbol IL8
Synonyms IL8; interleukin 8; interleukin-8; IL-8; C-X-C motif chemokine 8; alveolar macrophage chemotactic factor I; alveolar macrophage-derived chemotactic factor-I; CXCL8; AMCF-I;
Gene ID 396880
mRNA Refseq NM_213867
Protein Refseq NP_999032

Not For Human Consumption!

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