Active Recombinant Rabbit CXCL8 protein, His-tagged

Cat.No. : CXCL8-887R
Product Overview : Active Recombinant Rabbit CXCL8 protein(P19874)(23~101 aa), fused with N-terminal His tag, was expressed in E.coli.
  • Specification
  • Gene Information
  • Related Products
  • Download
Species : Rabbit
Source : E.coli
Tag : His
Protein Length : 23-101 aa
Form : Lyophilized from sterile 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Bio-activity : Interleukin 8 (IL8 or chemokine (C-X-C motif) ligand 8, CXCL8) is a chemokineproduced by macrophages and other cell types such as epithelial cells, airwaysmooth muscle cells and endothelial cells. Thus, chemotaxis assay used24-well microchemotaxis system was undertaken to detect the chemotactic effect of IL8onthe human T-lymphocyte leukemia cell line Jurkat. Briefly, Jurkat cellswereseeded into the upper chambers (100uL cell suspension, 106cells/ml in RPMI 1640with FBS free) and IL8 (0.1ng/mL, 1ng/mL and 10ng/mL diluted separatelyinserum free RPMI 1640 ) was added in lower chamber with a polycarbonatefilter(8um pore size) used to separate the two compartments. After incubationat 37°C with 5% CO2 for 1h, the filter was removed, then cells in lowchamber wereobserved by inverted microscope at low magnification (×100) and the number of migrated cells were counted at high magnification (×400) randomly (five fields for each filter). Result shows IL8 is able to induce migration of Jurkat cells. Themigrated Jurkat cells in low chamber at low magnification (×100) were shown. Five fields of each chamber were randomly chosen, and the migratedcells were counted at high magnification (×400). The optimum chemotax is of IL8 occurs at 1~10ng/mL.
Molecular Mass : 12.5kDa
Purity : > 95% as determined by SDS-PAGE.
Storage : Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
Reconstitution : Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Not For Human Consumption!

Inquiry

  • Reviews (0)
  • Q&As (0)

Customer Reviews

Write a review

Ask a Question for All CXCL8 Products

Required fields are marked with *

My Review for All CXCL8 Products

Required fields are marked with *

0
cart-icon
0
compare icon